The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 22, 2013

Filed:

Aug. 01, 2005
Applicants:

Wolfgang Weber, Berlin, DE;

Franz Paul Armbruster, Bensheim, DE;

Inventors:

Wolfgang Weber, Berlin, DE;

Franz Paul Armbruster, Bensheim, DE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

Method for the quantitative determination of vitamins, amino acids, or other substances necessary for life, in a substance mixture, whereby in the culture container, the cavities of a microtitration plate, in each case a predetermined number of vital cells of a suitable microorganism are prepared in a permanent manner. For this purpose, the cells are shock frozen at −80° C. and then freeze dried in a freezing solution containing 200 to 500 mM trehalose/sucrose. The freezing solution is preferably the test medium. The culture containers are preferably the wells of a microtitration plate.


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