The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 01, 2013
Filed:
May. 21, 2008
Peter Matthiessen, Vienna, AT;
Stefan Romeder-finger, Vienna, AT;
Peter Turecek, Klosterneuburg, AT;
Hans-peter Schwarz, Vienna, AT;
Peter Matthiessen, Vienna, AT;
Stefan Romeder-Finger, Vienna, AT;
Peter Turecek, Klosterneuburg, AT;
Hans-Peter Schwarz, Vienna, AT;
Baxter International Inc., Deerfield, IL (US);
Baxter Healthcare S.A., Glattpark (Opfikon), CH;
Abstract
Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on CAPTO MMC™ (mixed cation exchange/hydrophobic interaction gel) resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after CAPTO MMC™ (mixed cation exchange/hydrophobic interaction gel) chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.