The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 25, 2012
Filed:
Apr. 17, 2009
Shizunobu Igimi, Tokyo, JP;
Minoru Fujimori, Ibaraki, JP;
Shun'ichiro Taniguchi, Nagano, JP;
Michihiko Harada, Nagano, JP;
Takayuki Sasaki, Nagano, JP;
Akinobu Kajikawa, Yokohama, JP;
Shizunobu Igimi, Tokyo, JP;
Minoru Fujimori, Ibaraki, JP;
Shun'ichiro Taniguchi, Nagano, JP;
Michihiko Harada, Nagano, JP;
Takayuki Sasaki, Nagano, JP;
Akinobu Kajikawa, Yokohama, JP;
Anaeropharma Science, Inc., Tokyo, JP;
Abstract
The present invention provides an obligately anaerobic lactic acid bacterium having no risk of causing side effects in normal tissue and promising as a safe therapeutic agent and a gene transporter for a disease that is in an anaerobic environment such as a solid tumor, a preparation method therefor, and an expression vector useful in the preparation method. The obligately anaerobic lactic acid bacterium of the present invention has been artificially mutated from being facultatively anaerobic to being obligately anaerobic and, furthermore, is capable of being transformed by an expression vector having introduced thereinto a gene for expressing an active protein useful for the treatment of a disease that is in an anaerobic environment. Furthermore, the expression vector of the present invention functions in an obligately anaerobic lactic acid bacterium and contains a-derived plasmid replication protein gene (Rep), a secretion signal sequence (PslpA-SSartP) comprising a-derived s-layer gene promoter and a-derived PrtP protein secretion signal, and one or more selection marker genes. The expression vector of the present invention enables producing an extremely safe and excellent therapeutic agent and gene transporting microorganism for a disease that is in an anaerobic environment.