The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 11, 2012
Filed:
May. 15, 2009
Michalis Nicolaou, San Diego, CA (US);
Frederick Charles Bancroft, Carlsbad, CA (US);
John Patrick Herich, Cardiff, CA (US);
Swati Gupta, San Diego, CA (US);
Aung Naing Lwin, Carlsbad, CA (US);
Michalis Nicolaou, San Diego, CA (US);
Frederick Charles Bancroft, Carlsbad, CA (US);
John Patrick Herich, Cardiff, CA (US);
Swati Gupta, San Diego, CA (US);
Aung Naing Lwin, Carlsbad, CA (US);
Amylin Pharmaceuticals, LLC, San Diego, CA (US);
Astrazeneca Pharmaceuticals LP, Wilmington, DE (US);
Abstract
Provided herein are cell-based bioassays for measuring GLP-I receptor agonist activity of test compounds, such as GLP-I receptor agonist compounds. Exemplary GLP-I receptor agonist compounds include exendins, exendin analogs, GLP-1(7-37), and GLP-1(7-37) analogs. The bioassays are useful for quantitatively determining cAMP generated in samples containing GLP-I receptor agonist compounds (e.g., exenatide) and 6-23 (clone 6) cells having GLP-I receptors, whereby the amount of cAMP generated can be correlated to the GLP-I receptor agonist activity of the GLP-I receptor agonist compounds (e.g., exenatide). Suitable cell-based bioassays include enzyme-linked immunosorbent assays and homogeneous time-resolved fluorescence assays.