The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 04, 2012

Filed:

Apr. 07, 2009
Applicants:

Sha-sha Wang, Cockeysville, MD (US);

Keith Thornton, Pikesville, MD (US);

James G. Nadeau, Ellicott City, MD (US);

Tobin J. Hellyer, Owings Mills, MD (US);

Inventors:

Sha-Sha Wang, Cockeysville, MD (US);

Keith Thornton, Pikesville, MD (US);

James G. Nadeau, Ellicott City, MD (US);

Tobin J. Hellyer, Owings Mills, MD (US);

Assignee:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

The invention employs an unlabeled signal primer comprising a 5' adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.


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