The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 27, 2012
Filed:
Oct. 11, 1999
Anke Rattenholl, Halle, DE;
Adelbert Grossmann, Eglfing, DE;
Elisabeth Schwarz, Halle, DE;
Rainer Rudolph, Halle, DE;
Anke Rattenholl, Halle, DE;
Adelbert Grossmann, Eglfing, DE;
Elisabeth Schwarz, Halle, DE;
Rainer Rudolph, Halle, DE;
Scil Proteins GmbH, Halle, DE;
Abstract
The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.