The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 13, 2012
Filed:
Feb. 15, 2011
Applicants:
Marcus Hartmann, Muenster, DE;
Andre Broermann, Muenster, DE;
Inventors:
Marcus Hartmann, Muenster, DE;
Andre Broermann, Muenster, DE;
Assignee:
Cilian AG, Muenster, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/37 (2006.01);
U.S. Cl.
CPC ...
Abstract
A method for identifying a protease secretion deficient strain of a microorganism involves producing a mutant of the microorganism, followed by adding the mutant to gel-filled wells of a microtitration plate, incubating the mutant in the gel-filled wells under conditions call sing the mutant to secrete proteins, separating the mutant from the gel, and measuring activity of a secretion protease of the microorganism in the gel, wherein either (i) the gel is a substrate for the secretion protease or (ii) the gel contains a substrate for the secretion protease.