The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 06, 2012
Filed:
May. 25, 2011
Ilaria Adamo, Fagagna, IT;
Cecile Dueymes, Rodez, FR;
Andreas Schonberger, Muden/Aller, DE;
Jean-louis Imbach, Montpellier, FR;
Albert Meyer, Perols, FR;
Francois Morvan, Castelnau le Lez, FR;
Francoise Debart, Combaillaux, FR;
Jean-jacques Vasseur, Combaillaux, FR;
Meinolf Lange, Halle/Westf., DE;
Fritz Link, Bergisch-Gladbach, DE;
Ilaria Adamo, Fagagna, IT;
Cecile Dueymes, Rodez, FR;
Andreas Schonberger, Muden/Aller, DE;
Jean-Louis Imbach, Montpellier, FR;
Albert Meyer, Perols, FR;
Francois Morvan, Castelnau le Lez, FR;
Francoise Debart, Combaillaux, FR;
Jean-Jacques Vasseur, Combaillaux, FR;
Meinolf Lange, Halle/Westf., DE;
Fritz Link, Bergisch-Gladbach, DE;
Girindus AG, Bergisch-Gladbach, DE;
Centre National de la Recherche Scientifique, Paris Cedex, FR;
University of Montpellier II, Montpellier Cedex, FR;
Abstract
A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3'-protected compound of formula: with a nucleotide derivative having a 5′-protection group, or reacting a 5′-protected compound of formula with a nucleotide derivative having a 3′-protection group.