The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 30, 2012
Filed:
Dec. 06, 2007
Uma Sankararaman, Navi Mumbai, IN;
Dipanwita Maiti, Mumbai, IN;
Meera Sankarankutty, Mumbai, IN;
Rakesh Shekhawat, Dombivali(E), IN;
Narasimha Kumar Kopparapu, Andhra Pradesh, IN;
Gulnaz Zaidi, Maharshtra, IN;
Bipinchandra Rathod, Mumbai, IN;
Niren Praful Thakar, Mumbai, IN;
Priti Thakur, Mumbai, IN;
Anjali Chutke, Mumbai, IN;
Shrikant Mishra, Mumbai, IN;
Uma Sankararaman, Navi Mumbai, IN;
Dipanwita Maiti, Mumbai, IN;
Meera Sankarankutty, Mumbai, IN;
Rakesh Shekhawat, Dombivali(E), IN;
Narasimha Kumar Kopparapu, Andhra Pradesh, IN;
Gulnaz Zaidi, Maharshtra, IN;
Bipinchandra Rathod, Mumbai, IN;
Niren Praful Thakar, Mumbai, IN;
Priti Thakur, Mumbai, IN;
Anjali Chutke, Mumbai, IN;
Shrikant Mishra, Mumbai, IN;
USV Limited, Govandi Mumbai, Maharashtra, IN;
Abstract
The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragment as set forth in SEQ. ID. No.:1 encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partner consisting of 41 amino acids belonging toβ-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transformingwith the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP-HPLC to yield a target protein of ≧99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).