The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 16, 2012
Filed:
Nov. 18, 2004
José Remacle, Malonne, BE;
Isabelle Alexandre, Namur, BE;
Sylvain Margaine, Namur, BE;
Dieter Husar, Namur, BE;
José Remacle, Malonne, BE;
Isabelle Alexandre, Namur, BE;
Sylvain Margaine, Namur, BE;
Dieter Husar, Namur, BE;
Eppendorf Array Technologies S.A., Namur, BE;
Abstract
The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (), a support () having fixed upon its surface a micro-array comprising at least 5 capture molecules () being immobilized in specifically localized areas () of said support; introducing said labeled target molecules solution () into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light () from a light source () on the surface of the micro-array; measuring the electromagnetic light emission () from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.1 μmand about 10 mmand wherein each of the at least 4 localized areas is monitored with time with at least two measurements being done per localized area (), and processing and storing the values of the different measurements and quantifying at least 4 different target molecules present in solution using at least one measurement value for each said target.