The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 18, 2012
Filed:
Jun. 27, 2008
Applicants:
Yasushi Ikarashi, Inashiki-gun, JP;
Zenji Kawakami, Inashiki-gun, JP;
Inventors:
Yasushi Ikarashi, Inashiki-gun, JP;
Zenji Kawakami, Inashiki-gun, JP;
Assignee:
Tsumura & Co., Tokyo, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01); C12P 21/06 (2006.01); C07K 1/00 (2006.01);
U.S. Cl.
CPC ...
Abstract
To find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, it is intended to provide a method of bioassaying yokukansan characterized by comprising adding a yokukansan-containing test sample to astroglial cells to be cultivated under the condition of thiamine deficiency, and then determining the pharmacological activity value of yokukansan based on the glutamic acid or neutral red intake level in the cultivated astroglial cells.