The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 18, 2012

Filed:

Sep. 20, 2005
Applicants:

Shin-ichiro Imai, St. Louis, MO (US);

Javier R. Revollo, St. Louis, MO (US);

Andrew A. Grimm, St. Louis, MO (US);

Inventors:

Shin-Ichiro Imai, St. Louis, MO (US);

Javier R. Revollo, St. Louis, MO (US);

Andrew A. Grimm, St. Louis, MO (US);

Assignee:

Washington University, Saint Louis, MO (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/36 (2006.01); C07H 21/04 (2006.01); G01N 33/573 (2006.01); C12N 15/63 (2006.01); C12N 5/10 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention generally relates to a nicotinamide adenine dinucleotide (NAD) biosynthesis system and methods of screening for NAD biosynthesis effectors. Among the various aspects of the present invention is the provision of an in vitro-reconstituted mammalian NAD biosynthesis system that can be used for the high-throughput screening of chemical activators and inhibitors for mammalian NAD biosynthesis. Another aspect of the invention provides a method of identifying a compound that effects in vivo activity of NAD metabolic enzymes. Further aspects of the invention include nucleic acid sequences, vectors, and transformed cells that can be used in the methods described herein.


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