The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 28, 2012
Filed:
Jan. 12, 2005
Isabelle Meynial-salles, Fourquevaux, FR;
Benjamin Gonzalez, Riom, FR;
Philippe Soucaille, Deyme, FR;
Isabelle Meynial-Salles, Fourquevaux, FR;
Benjamin Gonzalez, Riom, FR;
Philippe Soucaille, Deyme, FR;
Metabolic Explorer, Saint Beauzire, FR;
Abstract
The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved '1,2-propanediol synthase activity', the resulting strain or strains of evolved micro-organisms possessing an improved '1,2-propanediol synthase activity' then being selected and isolated. The invention also concerns the initial micro-organisms and the evolved micro-organisms thus obtained, and a method for the preparation of 1,2-propanediol and possibly acetone by culture of said evolved micro-organisms.