The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 21, 2012

Filed:

Jul. 25, 2011
Applicants:

Eric Chen, Research Triangle Park, NC (US);

Cheryl M. Defontes, Research Triangle Park, NC (US);

Inventors:

Eric Chen, Research Triangle Park, NC (US);

Cheryl M. Defontes, Research Triangle Park, NC (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.


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