The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 24, 2012
Filed:
Jul. 27, 2009
Sally Ann Miller, Wooster, OH (US);
Gireesh Rajashekara, Wooster, OH (US);
Xiulan Xu, Wooster, OH (US);
Sally Ann Miller, Wooster, OH (US);
Gireesh Rajashekara, Wooster, OH (US);
Xiulan Xu, Wooster, OH (US);
The Ohio State University Research Foundation, Columbus, OH (US);
Abstract
subsp.(Cmm) is an important Gram-positive-bacterial pathogen that infects tomato plants causing wilting and cankers and leading to severe economic losses in commercial tomato production worldwide. In order to visualize the infection process of Cmm in germinating seeds, bioluminescent Cmm strains were constructed by transforming the lux reporter gene into the bacterium. A vector pXX2 was constructed by inserting a modified promoterless lux-operon to a Cmm transposon mutagenesis vector, pKGT452Cβ. After electroporation, pXX2 carrying the Cmx::luxABCDE::Tn1409 cassette resulted in insertion of lux-operon into the Cmm chromosome. The virulent, stable, constitutively bioluminescent strain BL-Cmm17 was selected for further characterization of growth properties and inoculation on tomato seeds. Using this bioluminescent strain, Cmm colonization dynamics of germinating seeds were monitored in real-time using the in vivo imaging system (IVIS).