The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 26, 2012
Filed:
Aug. 29, 2007
Hiroshi Nojima, Suita, JP;
Takahiro Tougan, Suita, JP;
Daisuke Okuzaki, Suita, JP;
Osaka University, Osaka, JP;
Abstract
A method for amplifying a trace amount of mRNA includes adding a dummy RNA to a solution containing the trace amount of mRNA, so as to prepare a mixed solution; synthesizing an anti-sense DNA by reverse transcription, which uses the mixed solution as a template; synthesizing a sense DNA which is complementary to the anti-sense DNA thus synthesized, so as to generate a double-strand DNA made of the sense DNA and the anti-sense DNA; ligating an RNA polymerase promoter sequence to the double-strand DNA thus generated, on a sense DNA 5' end side of the double-strand DNA, so as to prepare a double-strand DNA for amplification; and amplifying, by using RNA polymerase, an RNA from the double-strand DNA for amplification.