The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 29, 2012
Filed:
Jun. 20, 2008
Applicants:
Ute Ehringer, Waghäusel, DE;
Eva Kohlstrung, Plankstadt, DE;
Inventors:
Ute Ehringer, Waghäusel, DE;
Eva Kohlstrung, Plankstadt, DE;
Assignee:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C12N 1/06 (2006.01); C12N 1/21 (2006.01); C07K 14/51 (2006.01);
U.S. Cl.
CPC ...
Abstract
A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine.