The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 15, 2012
Filed:
Nov. 22, 2002
Applicants:
Nobuyoshi Shimizu, Tokyo, JP;
Atsushi Takayanagi, Tokyo, JP;
Michiyo Okui, Tokyo, JP;
Inventors:
Assignee:
Keio University, , JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/04 (2006.01); C12P 21/08 (2006.01); C12Q 1/70 (2006.01); C12N 15/00 (2006.01); A61K 39/00 (2006.01); A61K 39/395 (2006.01);
U.S. Cl.
CPC ...
Abstract
An artificial antibody library with a super-repertory (10or more) is constructed by: using a cDNA library as a template, amplifying a fragment containing the CDR1 and CDR2s regions of the VH or VL region of immunoglobulin gene and a fragment containing the CDR3 region each by the PCR method; integrating the VH library and the VL library, which are little contaminated with unexpressionable repertory and have high safety, into an non-expression vector; transferring it into a host; and then shuffling the VH region in the VH library with the VL region in the VL library.