Reciprocal symmetry plots as a new representation of countercurrent chromatograms

Abstract

Various methods for visualization of output from a liquid-liquid chromatographic instrument are provided. One or more analytes detected by a liquid-liquid chromatographic instrument are visualized by providing a data set comprising a plurality of data points corresponding to one or more analytes detected by the instrument, wherein the data points comprise at least one parameter related to a K-value or a parameter from which a K-value can be determined. A K-value is calculated for at least a portion of the data set, and at least a portion of those K-values transformed by a reciprocal transformation to generate output data having a transformed K-value, wherein the transformed K-value is a real number for all K undergoing the transformation, thereby ensuring that all analytes detected by the instrument are plotted in a single chromatogram. The output data is provided to a user. The output data may be used for instrument performance testing, design, calibration, or for selecting suitable solvent systems to detect analytes of interest.