The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 08, 2012
Filed:
Nov. 18, 2010
Stefan W. Hell, Göttingen, DE;
Jonas Fölling, Heidelberg, DE;
Christian Eggeling, Göttingen, DE;
Alexander Egner, Einbeck, DE;
Andreas Schönle, Göttingen, DE;
Mariano Bossi, Buenos Aires, AR;
Stefan W. Hell, Göttingen, DE;
Jonas Fölling, Heidelberg, DE;
Christian Eggeling, Göttingen, DE;
Alexander Egner, Einbeck, DE;
Andreas Schönle, Göttingen, DE;
Mariano Bossi, Buenos Aires, AR;
Abstract
In high spatial resolution imaging, a structure in a specimen is marked with a substance which, in a first electronic state, is excited by light of one wavelength to emit fluorescent light, which is also converted from its first into a second electronic state by that light, and which returns from its second into its first electronic state. The specimen is imaged onto a sensor at a spatial resolution not resolving an average spacing between neighboring molecules of the substance, and exposed to the light at such an intensity that the molecules in the first state are alternately excited to emit fluorescent light and converted into their second state, and that at least 10% of the molecules presently in their first state lie at a distance from their closest neighboring molecules in their first state which is greater than the spatial resolution of the imaging onto the sensor.