The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 08, 2012
Filed:
Feb. 17, 2010
Joseph P. Vacanti, Winchester, MA (US);
Robert Rubin, Brookline, MA (US);
Wing Cheung, Swampscott, MA (US);
Jeffrey T. Borenstein, Newton, MA (US);
Joseph P. Vacanti, Winchester, MA (US);
Robert Rubin, Brookline, MA (US);
Wing Cheung, Swampscott, MA (US);
Jeffrey T. Borenstein, Newton, MA (US);
The General Hospital Corporation, Boston, MA (US);
The Charles Stark Draper Laboratory, Cambridge, MA (US);
Abstract
A method for detecting one or more metabolites of a test agent in a tissue including: A) incubating a test agent and an enzyme within a three-dimensional structure comprising a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, and wherein the first mold or polymer scaffold has microchannels that form a fluidic branching network that mimics the forces and transport of natural vasculatures and wherein the second mold or polymer scaffold comprises cells; B) forming an enzyme-substrate complex between the enzyme and the test agent; and C) detecting one or more metabolites of the test agent.