The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 01, 2012
Filed:
Mar. 21, 2008
Ira S. Cohen, Stony Brook, NY (US);
Amy B. Rosen, New York, NY (US);
Peter R. Brink, Setauket, NY (US);
Glenn Gaudette, Holden, MA (US);
Michael R. Rosen, New York, NY (US);
Richard B. Robinson, Cresskill, NJ (US);
Ira S. Cohen, Stony Brook, NY (US);
Amy B. Rosen, New York, NY (US);
Peter R. Brink, Setauket, NY (US);
Glenn Gaudette, Holden, MA (US);
Michael R. Rosen, New York, NY (US);
Richard B. Robinson, Cresskill, NJ (US);
The Trustees of Columbia University in the City of New York, New York, NY (US);
The Research Foundation of State University of New York, Albany, NY (US);
Abstract
The present invention provides methods and compositions relating to the labeling of target cells with nanometer scale fluorescent semiconductors referred to as quantum dots (QDs). Specifically, a delivery system is disclosed based on the use of negatively charged QDs for delivery of a tracking fluorescent signal into the cytosol of target cells via a passive endocytosis-mediated delivery process. In a specific embodiment of the invention the target cell is a stem cell, preferably a mesenchymal stem cell (MSC). Such labeled MSCs provide a means for tracking the distribution and fate of MSCs that have been genetically engineered to express, for example, a hyperpolarization-activated cyclic nucleotide-gated ('HCN') channel and administered to a subject to create a biological pacemaker. The invention is based on the discovery that MSCs can be tracked in vitro for up to at least 6 weeks. Additionally, QDs delivered in vivo can be tracked for up to at least 8 weeks, thereby permitting for the first time, the complete 3-D reconstruction of the locations of all MSCs following administration into a host.