The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 01, 2012

Filed:

Nov. 05, 2010
Applicants:

Mark S. Payne, Wilmington, DE (US);

Stephen K. Picataggio, Solana Beach, CA (US);

Amy Kuang-hsu, Redwood City, CA (US);

Ramesh Velayudhan Nair, Cupertino, CA (US);

Fernando Valle, Burlingame, CA (US);

Philippe Soucaille, Deyme, FR;

Donald Eugene Trimbur, Palo Alto, CA (US);

Inventors:

Mark S. Payne, Wilmington, DE (US);

Stephen K. Picataggio, Solana Beach, CA (US);

Amy Kuang-Hsu, Redwood City, CA (US);

Ramesh Velayudhan Nair, Cupertino, CA (US);

Fernando Valle, Burlingame, CA (US);

Philippe Soucaille, Deyme, FR;

Donald Eugene Trimbur, Palo Alto, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C12N 15/64 (2006.01); C12N 1/20 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.


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