The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 24, 2012
Filed:
Sep. 22, 2008
Angelo P. Consalvo, Monroe, NY (US);
Nozer M. Mehta, Randolph, NJ (US);
William Stern, Tenafly, NJ (US);
James P. Gilligan, Union, NJ (US);
Angelo P. Consalvo, Monroe, NY (US);
Nozer M. Mehta, Randolph, NJ (US);
William Stern, Tenafly, NJ (US);
James P. Gilligan, Union, NJ (US);
Unigene Laboratories, Inc., Boonton, NJ (US);
Abstract
Conversion in vitro of X-Gly to X-alpha-hydroxy-Gly or X-NH(X being a peptide or any other compound having a carbonyl group capable of forming a covalent bond with glycine) is accomplished enzymatically in the presence of keto acids, or salts or esters thereof, to provide a good yield without the necessity of catalase or similar enzymatic reaction enhancers. Peptidylglycine α-amidating monooxygenase (PAM) is a preferred enzyme for catalyzing the conversion. Alternatively, peptidylglycine α-hydroxylating monooxygenase (PHM) is utilized to convert X-Gly to X-alpha-hydroxy-Gly which may be recovered, or optionally may be simultaneously or sequentially converted to an amide by either a Lewis base or action of the enzyme peptidyl α-hydroxyglycine α-amidating lyase (PAL). Both PHM and PAL are functional domains of PAM.