The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 24, 2012
Filed:
Oct. 19, 2006
Masashi Urabe, Tochigi, JP;
Keiya Ozawa, Tochigi, JP;
Saskia Jacoba Petronella Haast, Huizen, NL;
Wilhelmus Theodorus Johannes Maria Christiaan Hermens, Almere, NL;
Masashi Urabe, Tochigi, JP;
Keiya Ozawa, Tochigi, JP;
Saskia Jacoba Petronella Haast, Huizen, NL;
Wilhelmus Theodorus Johannes Maria Christiaan Hermens, Almere, NL;
Amsterdam Molecular Therapeutics B.V., Amsterdam, NL;
Abstract
The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity of the viral particles.