The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 24, 2012
Filed:
Mar. 01, 2005
Hughes Jaccard, Yverdon-les-Bains, CH;
Kai Johnsson, Lausanne, CH;
Maik Kindermann, Frankfurt, DE;
India Christina Sielaff, Lausanne, CH;
Hughes Jaccard, Yverdon-les-Bains, CH;
Kai Johnsson, Lausanne, CH;
Maik Kindermann, Frankfurt, DE;
India Christina Sielaff, Lausanne, CH;
Ecole Polytechnique Federale de Lausanne, Lausanne, CH;
Abstract
The invention relates to substrates for O-alkylguanine-DNA alkyltransferases (AGT) of formula R-A-X—CH—R—R-L, wherein A is a group recognized by AGT as a substrate, X is oxygen or sulfur, Ris a group —R-Lor a group R, Rand Rare, independently of each other, a linker, Ris an aromatic or a heteroaromatic group, or an optionally substituted unsaturated alkyl, cycloalkyl or heterocyclyl group with the double bond connected to CH, Ris arylmethyl or heteroarylmethyl or an optionally substituted cycloalkyl, cycloalkenyl or heterocyclyl group, Lis a label, a plurality of same or different labels, a bond connecting Rto A forming a cyclic substrate, or a further group —R—CH—X-A-R, and Lis a label or a plurality of same or different labels. The invention further relates to methods of transferring a label from these substrates to O-alkylguanine-DNA alkyltransferases (AGT) and AGT fusion proteins.