The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 10, 2012
Filed:
Jun. 24, 2009
Chun-cheng Lin, Hsinchu, TW;
Mu-lin Chen, Hsinchu, TW;
Po-chiao Lin, Hsinchu, TW;
Yu-ju Chen, Hsinchu, TW;
Shu-hua Chen, Hsinchu, TW;
Chun-Cheng Lin, Hsinchu, TW;
Mu-Lin Chen, Hsinchu, TW;
Po-Chiao Lin, Hsinchu, TW;
Yu-Ju Chen, Hsinchu, TW;
Shu-Hua Chen, Hsinchu, TW;
National Tsing Hua University, Hsinchu, TW;
Abstract
A method for immobilizing glycoprotein is disclosed. A surface is provided. Next, a boronic acid is contacted to the surface. The boronic acid is represented as Y—R—B—(OH)and includes a boronic acid group (B—(OH)), a linker R, and a functional group Y, in which the boronic acid is bonded to the surface via the functional group Y. A glycoprotein is contacted to the boronic acid, wherein two spatially adjacent hydroxyl groups of a carbohydrate chain form a boronate ester with the boronic acid group of the boronic acid to immobilize the glycoprotein. The goal of site-specific and covalent protein immobilization without interfering the binding affinity of an antibody to antigen may be thus achieved with the reaction of boronic acid and the carbohydrate chain of Fc region.