The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 03, 2012
Filed:
Jul. 01, 2010
Richard M. West, Cardiff, GB;
Nigel Bosworth, Cardiff, GB;
Ratnakar B. Mujumdar, Wexford, PA (US);
GE Healthcare UK Limited, Little Chalfont, GB;
Carnegie Mellon University, Pittsburgh, PA (US);
Abstract
Disclosed are cyanine dyes that are useful for labelling and detecting biological and other materials. The dyes are of formula (I): in which groups Rand Rare attached to the Zring structure and groups Rand Rare attached to the Zring structure, and n=1, 2 or 3; Zand Zindependently represent the carbon atoms necessary to complete a one ring, or two-fused ring aromatic system; at least one of groups R, R, R, R, R, Rand Ris the group -E-F where E is a single bond or a spacer group and F is a target bonding group; one or more of groups R, R, Rand Rare independently selected from the group —(CH)—W, where W is sulphonic acid or phosphonic acid and k is an integer from 1 to 10. The dyes may be used in fluorescence labelling applications, where the presence of one and preferably multiple water solubilizing groups attached to the 3-position of the indolinium ring reduces dye-dye interactions, and hence dye-dye quenching, particularly where multiple dye molecules are attached to components such as nucleic acids, oligonucleotides, proteins and antibodies.