The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 03, 2012
Filed:
May. 18, 2007
Applicants:
Yoshikazu Fujii, Iwata, JP;
Tomohiro Tamura, Sapporo, JP;
Inventors:
Yoshikazu Fujii, Iwata, JP;
Tomohiro Tamura, Sapporo, JP;
Assignees:
Microbiopharm Japan Co., Ltd., Tokyo, JP;
National Institute of Advanced Industrial Science and Technology, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 15/00 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract
A vitamin Dhydroxylase is purified fromcell, and a primer is designed based on amino acid sequence obtained from hydroxylase. Subsequently, PCR is conducted using genomic DNA ofas a template to clone a gene for the vitamin Dhydroxylase. By conducting a conversion reaction using a microorganism in which the vitamin Dhydroxylase gene is expressed using a proper expression system, a hydroxide of vitamin D or the like (e.g., hydroxy vitamin D) can be produced with high efficiency.