The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2012
Filed:
Nov. 03, 2010
Antoni Peters, Billerica, MA (US);
Philip Goddard, Billerica, MA (US);
John Charkoudian, Billerica, MA (US);
Neil Soice, Billerica, MA (US);
Dave Brewster, Billerica, MA (US);
Anja Dedeo, Billerica, MA (US);
Antoni Peters, Billerica, MA (US);
Philip Goddard, Billerica, MA (US);
John Charkoudian, Billerica, MA (US);
Neil Soice, Billerica, MA (US);
Dave Brewster, Billerica, MA (US);
Anja Dedeo, Billerica, MA (US);
EMD Millipore Corporation, Billerica, MA (US);
Abstract
Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.