The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2012
Filed:
Jul. 22, 2004
Jean-paul Di Rago, Sainte Helene, FR;
Nathalie Bonnefoy, Gif-sur-Yvette, FR;
Stéphane Duvezin-caubet, Bordeaux, FR;
Jean-Paul Di Rago, Sainte Helene, FR;
Nathalie Bonnefoy, Gif-sur-Yvette, FR;
Stéphane Duvezin-Caubet, Bordeaux, FR;
Centre National de la Recherche Scientifique, Paris, FR;
Universite Victor Segalen Bordeaux 2, Bordeaux, FR;
Abstract
The invention relates to an industrial method for producing an interest heterologous RNA and to a system for carrying out said production method consisting (1) in transforming mitochondrions of yeast cells free of mitochondrial RNA with a mitochondrial transcription vector comprising at least one copy of the DNA encoding said interest heterologous RNA which are controlled by regulatory element(s) of the mitochondrial transcription and a reporter gene thereof or a fragment of said reporter gene, 2) identifying yeast transformants by incorporating the interest DNA into the mitochondrions thereof, (3) culturing the yeast mitochondrial transformants selected at the stage (2), (4) isolating the mitochondrions from the yeast mitochondrial transformants obtainable at stages (3) and in extracting and purifying the interest heterologous RNA from said mitochondrions.