The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 20, 2012
Filed:
Oct. 30, 2006
Koichi Shimizu, Sapporo, JP;
Takeshi Kawaguchi, Tokyo, JP;
Yasuyo Maeda, Tokyo, JP;
Takehide Matsuda, Tokyo, JP;
Koichi Shimizu, Sapporo, JP;
Takeshi Kawaguchi, Tokyo, JP;
Yasuyo Maeda, Tokyo, JP;
Takehide Matsuda, Tokyo, JP;
National University Corporation Hokkaido University, Sapporo-shi, Hokkaido, JP;
Seiken Co., Ltd., Tokyo, JP;
Aska Special Laboratory Co., Ltd., Kawasaki-shi, Kanagawa, JP;
Abstract
A chemiluminescent enzyme immunoassay method whereby a target substance such as a protein is assayed. This chemiluminescent enzyme immunoassay method comprises: the step of capturing an immune complex containing an enzyme-labeled antibody, which is labeled with an enzyme acting a chemiluminescent substrate, and the target substance on a support having no solution layer; the step of overlaying a support membrane containing the chemiluminescent substrate on the immune complex having been captured above; and the step of measuring the luminescence dose caused by the reaction between the enzyme-labeled antibody and the chemiluminescent substrate to thereby quantify the target substance. Since a highly sensitive chemiluminescent enzyme immunoassay is conducted by using a non-liquid phase type reaction system in the chemiluminescent enzyme immunoassay method as described above, multiple items can be assayed by using only a small amount of a specimen and, furthermore, the target substance can be assayed at a high sensitivity thereby without resorting to any troublesome procedures such as pipetting a reagent.