The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 20, 2012
Filed:
Apr. 11, 2011
Alan Sloma, Davis, CA (US);
Leslie Naggiar, Legal Representative, Suffern, NY (US);
Regine Behr, Roseville, CA (US);
William Widner, Davis, CA (US);
Maria Tang, Fairfield, CA (US);
David Sternberg, Davis, CA (US);
Linda Sternberg, Legal Representative, Davis, CA (US);
Stephen Brown, Davis, CA (US);
Alan Sloma, Davis, CA (US);
Leslie Naggiar, legal representative, Suffern, NY (US);
Regine Behr, Roseville, CA (US);
William Widner, Davis, CA (US);
Maria Tang, Fairfield, CA (US);
David Sternberg, Davis, CA (US);
Linda Sternberg, legal representative, Davis, CA (US);
Stephen Brown, Davis, CA (US);
Novozymes, Inc., Davis, CA (US);
Abstract
The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating ahost cell under conditions suitable for production of the hyaluronic acid, wherein thehost cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.