The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2012
Filed:
Apr. 06, 2009
Xiaomeng Shi, West Roxbury, MA (US);
Muriel Medard, Belmont, MA (US);
Ralf Koetter, Munich, DE;
Nuala Ann Koetter, Legal Representative, Munich, DE;
Desmond S. Lun, Mawson Lakes, AU;
Xiaomeng Shi, West Roxbury, MA (US);
Muriel Medard, Belmont, MA (US);
Ralf Koetter, Munich, DE;
Nuala Ann Koetter, legal representative, Munich, DE;
Desmond S. Lun, Mawson Lakes, AU;
Massachusetts Institute of Technology, Cambridge, MA (US);
Abstract
In some aspects, a method of automated base-calling using at least one image obtained from a chemical sequencing process performed simultaneously on a plurality of DNA strands, the at least one image including intensity information corresponding to locations of at least one base in the plurality of DNA strands is provided. The method comprises processing the at least image to obtain a function corresponding to the intensity information in the at least one image for the at least one base, the function incorporating intensity information corresponding to each of the plurality of DNA strands, identifying a plurality of peaks in the function, the plurality of peaks indicating possible locations for the at least one base in the plurality of DNA strands, assigning membership to each of the plurality of peaks by determining whether each of the plurality of peaks is believed to have resulted from none, one or multiple of the plurality of DNA strands, and computing a sequence for the at least one base for each of the plurality of DNA strands based, at least in part, on the membership assignment.