The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 28, 2012

Filed:

Jun. 01, 2007
Applicants:

Vijay M. Krishnamurthy, Cambridge, MA (US);

Lara A. Estroff, Dryden, NY (US);

Vincent Semetey, Paris, FR;

Samuel W. Thomas, Boston, MA (US);

George K. Kaufman, Somerville, MA (US);

Zihni Basar Bilgicer, Cambridge, MA (US);

George M. Whitesides, Newton, MA (US);

Inventors:

Vijay M. Krishnamurthy, Cambridge, MA (US);

Lara A. Estroff, Dryden, NY (US);

Vincent Semetey, Paris, FR;

Samuel W. Thomas, Boston, MA (US);

George K. Kaufman, Somerville, MA (US);

Zihni Basar Bilgicer, Cambridge, MA (US);

George M. Whitesides, Newton, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/00 (2006.01); C07K 1/22 (2006.01); C07K 1/30 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment.


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