The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 14, 2012

Filed:

Jun. 20, 2007
Applicants:

Ramakrishnan Melarkode, Bangalore, IN;

Akundi Venkata Sriram, Bangalore, IN;

Kedarnath Nanjund Sastry, Bangalore, IN;

Lakshmi Prabha Varadarajalu, Bangalore, IN;

Shrikumar Suryanarayan, Bangalore, IN;

Inventors:

Ramakrishnan Melarkode, Bangalore, IN;

Akundi Venkata Sriram, Bangalore, IN;

Kedarnath Nanjund Sastry, Bangalore, IN;

Lakshmi Prabha Varadarajalu, Bangalore, IN;

Shrikumar Suryanarayan, Bangalore, IN;

Assignee:

Biocon Limited, Bangalore, Karnataka, IN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/09 (2006.01); C12N 15/74 (2006.01); C12P 21/06 (2006.01); C12P 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention relates to a method of producing a biologically active polypeptide having insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified host cell that has protease gene knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed host cell to produce the biologically active polypeptide; and a method of producing a biologically active polypeptide having an N-terminal recognition site His-Gly with insulinotropic activity, the method comprising steps of: (a) transforming a genetically modifiedthat has protease gene STE13 knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformedto produce the biologically active polypeptide.


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