The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 17, 2012

Filed:

Apr. 06, 2010
Applicants:

John J. Donnelly, Moraga, CA (US);

Kimberly S. Denis-mize, Concord, CA (US);

Gary S. Ott, Oakland, CA (US);

Inventors:

John J. Donnelly, Moraga, CA (US);

Kimberly S. Denis-Mize, Concord, CA (US);

Gary S. Ott, Oakland, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C12N 15/63 (2006.01); C12N 5/0783 (2010.01); C12N 5/0784 (2010.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides an effective method for the transfection of dendritic cells by non-viral methods. The present invention provides this benefit by incubating dendritic cells and a specified transfection agent. The transfection agent comprises a polynucleotide and microparticles, with the microparticles being comprised of biodegradable polymer and cationic detergent. The dendritic cells and transfection agent are incubated for a time sufficient to transfect the dendritic cells with the polynucleotide.


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