The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 2012
Filed:
Jul. 09, 2010
Carl T. Wittwer, Salt Lake City, UT (US);
Gudrun Reed, Salt Lake City, UT (US);
Virginie Dujols, Sandy, UT (US);
Luming Zhou, Salt Lake City, UT (US);
Carl T. Wittwer, Salt Lake City, UT (US);
Gudrun Reed, Salt Lake City, UT (US);
Virginie Dujols, Sandy, UT (US);
Luming Zhou, Salt Lake City, UT (US);
University of Utah Research Foundation, Salt Lake City, UT (US);
Idaho Technology, Inc., Salt Lake City, UT (US);
Abstract
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.