The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 06, 2011
Filed:
Dec. 11, 2008
Ronald W. Davis, Palo Alto, CA (US);
Stefanie S. Jeffrey, Los Altos Hills, CA (US);
Michael N. Mindrinos, Menlo Park, CA (US);
R. Fabian Pease, Stanford, CA (US);
Ashley Ann Powell, Mountain View, CA (US);
Amirali Hajhossein Talasaz, Los Altos, CA (US);
Ronald W. Davis, Palo Alto, CA (US);
Stefanie S. Jeffrey, Los Altos Hills, CA (US);
Michael N. Mindrinos, Menlo Park, CA (US);
R. Fabian Pease, Stanford, CA (US);
Ashley Ann Powell, Mountain View, CA (US);
AmirAli Hajhossein Talasaz, Los Altos, CA (US);
The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA (US);
Abstract
Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.