The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 06, 2011
Filed:
Aug. 08, 2008
Applicants:
Toshiaki Banzai, Fukuoka-ken, JP;
Yukiko Koyama, Fukuoka-ken, JP;
Shuji Sonezaki, Fukuoka-ken, JP;
Inventors:
Assignee:
Toto Ltd., Fukuoka-ken, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
Abstract
The present invention relates to a method of expressing an objective protein at a high level and stably as well as for a long period even in the absence of a selection drug with a recombinant mammal cell. More particularly, the present invention relates to a method of producing an objective protein by providing a recombinant mammal cell having multiple copies of the exogenous objective protein gene expression unit integrated into a hypoxanthine-phosphoribosyl transferase enzyme (hprt) gene locus and culturing said cell.