The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 06, 2011
Filed:
Sep. 28, 2010
Vladimir L. Makarov, Ann Arbor, MI (US);
Emmanuel Kamberov, Ann Arbor, MI (US);
Brendan J. Tarrier, Belleville, MI (US);
Vladimir L. Makarov, Ann Arbor, MI (US);
Emmanuel Kamberov, Ann Arbor, MI (US);
Brendan J. Tarrier, Belleville, MI (US);
Rubicon Genomics, Inc., Ann Arbor, MI (US);
Abstract
The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.