The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 29, 2011

Filed:

Mar. 28, 2002
Applicants:

Robert D. Rosenberg, Cambridge, MA (US);

Lijuan Zhang, St. Charles, MO (US);

David L. Beeler, Cambridge, MA (US);

Inventors:

Robert D. Rosenberg, Cambridge, MA (US);

Lijuan Zhang, St. Charles, MO (US);

David L. Beeler, Cambridge, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 11/00 (2006.01); C12P 19/04 (2006.01); C08B 37/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Disclosed are methods of 6-O-sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations may by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.


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