The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 18, 2011
Filed:
Sep. 28, 2010
Yuhiro Sakai, Itabashi-ku, JP;
Katsuyuki Yamanaka, Itabashi-ku, JP;
Mika Takeda, Hiroshima, JP;
Tomohisa Okura, Hiroshima, JP;
Koichiro Tsuji, Hiroshima, JP;
Yuhiro Sakai, Itabashi-ku, JP;
Katsuyuki Yamanaka, Itabashi-ku, JP;
Mika Takeda, Hiroshima, JP;
Tomohisa Okura, Hiroshima, JP;
Koichiro Tsuji, Hiroshima, JP;
GC Corporation, Tokyo, JP;
Two Cells Co., Ltd., Hiroshima-shi, JP;
Abstract
To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.