The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 11, 2011
Filed:
Sep. 28, 2010
Michael M. Becker, San Diego, CA (US);
Kristin W. Livezey, Encinitas, CA (US);
Wai-chung Lam, San Diego, CA (US);
Michael M. Becker, San Diego, CA (US);
Kristin W. Livezey, Encinitas, CA (US);
Wai-Chung Lam, San Diego, CA (US);
Gen-Probe Incorporated, San Diego, CA (US);
Abstract
Disclosed are methods for selective amplification of target nucleic acid sequence using a tagged oligonucleotide comprising a target hybridizing sequence that hybridizes to a 3'-end of the target nucleic acid and a tag sequence situated 5′ to the target hybridizing sequence. The tagged oligonucleotide is hybridized to the target nucleic acid and, after reducing the effective concentration of unhybridized tagged oligonucleotide having an active form, a primer extension reaction is initiated to produce a primer extension product. Further amplification also utilizes a first oligonucleotide that hybridizes to the 3′ end of the complement of the target nucleic acid and a second oligonucleotide that hybridizes to the complement of the tag sequence. Also disclosed are reaction mixtures for use in the disclosed methods comprising the tagged oligonucleotide hybridized to target nucleic and substantially free of an active form of unhybridized tagged oligonucleotide.