The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 04, 2011
Filed:
Aug. 06, 2007
Tomomi Kanehara, Kumamoto, JP;
Hiroyuki Yokote, Kumamoto, JP;
Kunio Ohkuma, Kumamoto, JP;
Masahiko Kuranaga, Kumamoto, JP;
Shigeru Morikawa, Musashimurayama, JP;
Tomomi Kanehara, Kumamoto, JP;
Hiroyuki Yokote, Kumamoto, JP;
Kunio Ohkuma, Kumamoto, JP;
Masahiko Kuranaga, Kumamoto, JP;
Shigeru Morikawa, Musashimurayama, JP;
Abstract
A safer live smallpox vaccine, which contains a lowered content of revertants, is provided. A process for manufacturing a live smallpox vaccine which comprises steps of: inoculating a master seed solution of an attenuated vaccinia virus to an appropriate number of containers (1 to n wherein n is an integer) of rabbit kidney cells and incubating them; inoculating a portion of the cultured solution obtained from each container to RK-13 cells and to Vero E6 cells and incubating them to thereby select containers which contain a cultured solution that forms plaques in RK-13 cells but not in Vero E6 cells; and preparing a drug substance of vaccine using the aforementioned cultured solution (working seed solution), and a live smallpox vaccine prepared in the aforementioned process.