The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 20, 2011

Filed:

Jun. 28, 2010
Applicants:

Marie Eskling, Horsholm, DK;

Klaus Gregorius Nielsen, Soborg, DK;

Inventors:

Marie Eskling, Horsholm, DK;

Klaus Gregorius Nielsen, Soborg, DK;

Assignee:

BN ImmunoTherapeutics, Inc., Mountain View, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/16 (2006.01); A61K 38/17 (2006.01); C07K 14/00 (2006.01); C07K 14/435 (2006.01); C07K 14/705 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), C) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.


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