The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 20, 2011
Filed:
Mar. 12, 2009
Scott E. Gygax, Bordentown, NJ (US);
John-paul Vermitsky, Philadelphia, PA (US);
Sean G. Chadwick, Florence, NJ (US);
Matthew J. Self, Westmont, NJ (US);
Eli Mordechai, Robbinsville, NJ (US);
Martin E. Adelson, Belle Mead, NJ (US);
Scott E. Gygax, Bordentown, NJ (US);
John-Paul Vermitsky, Philadelphia, PA (US);
Sean G. Chadwick, Florence, NJ (US);
Matthew J. Self, Westmont, NJ (US);
Eli Mordechai, Robbinsville, NJ (US);
Martin E. Adelson, Belle Mead, NJ (US);
Medical Diagnostic Laboratories, LLC, Hamilton, NJ (US);
Abstract
There is disclosed a method for determining azole resistance in. A biological sample containingis obtained and a normalized mRNA level of CDR1 gene is determined using qRT-PCR. Using a microbroth dilution assay conducted at azole concentrations of about 2-8 μg/mL, a susceptible isolate ofis obtained. A qRT-PCR assay is employed on the susceptible isolate and an average mRNA level of CDR1 is obtained. A fold-change value for CDR1 is obtained by comparing the CDR1 mRNA level of the biological sample with that of the average mRNA level. A ≧2-fold change value is indicative of an azole resistance in. The present method provides a qRT-PCR assay for azole resistance that has a sensitivity of ≧90% and a specificity of ≧90%.
Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile