The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 20, 2011
Filed:
Feb. 23, 2007
Charles Robert Bupp, Ii, Lafayette, CA (US);
K. Yeon Choi, Maplewood, MN (US);
Rachel Anne Holmes-davis, Richmond, CA (US);
Alexander Izmailov, Toronto, CA;
Heather Koshinsky, El Cerrito, CA (US);
Christopher Jon Nulf, Berkeley, CA (US);
Mickey Urdea, Alamo, CA (US);
Miaomiao Wang, El Cerrito, CA (US);
Brian David Warner, Martinez, CA (US);
Michael Zwick, Vacaville, CA (US);
Charles Robert Bupp, II, Lafayette, CA (US);
K. Yeon Choi, Maplewood, MN (US);
Rachel Anne Holmes-Davis, Richmond, CA (US);
Alexander Izmailov, Toronto, CA;
Heather Koshinsky, El Cerrito, CA (US);
Christopher Jon Nulf, Berkeley, CA (US);
Mickey Urdea, Alamo, CA (US);
Miaomiao Wang, El Cerrito, CA (US);
Brian David Warner, Martinez, CA (US);
Michael Zwick, Vacaville, CA (US);
Investigen, Inc., Hercules, CA (US);
Abstract
Methods of determining the presence or amount of a target polynucleotide in a sample are provided. A sample that contains a target polynucleotide, a polynucleotide that may include nucleic acid analogs and that is complementary to a target nucleic acid sequence of the target polynucleotide, and a dye for which the rate of change in an optical property is different in the presence and absence of a target polynucleotide/polynucleotide hybrid are combined to produce a reaction mixture. The rate of change in an optical property of the dye in the reaction mixture is compared to a reference value characteristic of the rate of change in the optical property of the dye in a similar reaction mixture containing a known amount of a polynucleotide/polynucleotide hybrid to determine a relative rate of change in the optical property. The relative rate of change in the optical property of dye in the reaction mixture is correlated with the presence or amount of the specified target polynucleotide in the sample.