The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 20, 2011
Filed:
Jun. 29, 2007
Srabani Bhaumik, Niskayuna, NY (US);
Eric Dustin Agdeppa, Fairview, NJ (US);
June Klimash, Niskayuna, NY (US);
Jeannette Christine Depuy, Watervliet, NY (US);
Srabani Bhaumik, Niskayuna, NY (US);
Eric Dustin Agdeppa, Fairview, NJ (US);
June Klimash, Niskayuna, NY (US);
Jeannette Christine DePuy, Watervliet, NY (US);
General Electric Company, Niskayuna, NY (US);
Abstract
Provided herein are methods for imaging an optically labeled target cell implanted in vivo using a reporter construct that encodes an enzyme that is not endogenous to the target cell. The disclosed methods include the steps of: (a) introducing target cell transformed with non-endogenous enzyme into a mammalian subject; (b) contacting the transformed target cell with a cell-permeable soluble fluorescent dye; and (c) observing a fluorescent signal generated by the target cell present in the mammalian subject. In some embodiments, the non-endogenous enzyme comprises a nitroreductase. In some embodiments, the methods may also include the step of tracking the fluorescent target cells in the mammalian subject over time.