The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 13, 2011
Filed:
Jul. 23, 2008
Dayang Izawati Bt. Abang Masli, Kajang, MY;
Ahmad Parveez Ghulam Kadir, Kajang, MY;
Abdul Masani Mat Yunus, Kajang, MY;
Dayang Izawati bt. Abang Masli, Kajang, MY;
Ahmad Parveez Ghulam Kadir, Kajang, MY;
Abdul Masani Mat Yunus, Kajang, MY;
Malaysian Palm Oil Board, Kajang, MY;
Abstract
A method for producing transgenic plants, including treating a target tissue using plasmolyzing media (PM) which contains 4% to 10% of sucrose and 100 μM to 300 μM of Acetosyringone (AS) and gold particles. The target tissue is infected by a bacterial suspension using a suitable strain and a suitable transformation vector. A PM containing 4% to 10% sucrose and 100 μM to 300 μM AS is treated for a period between 1 to 3 days. Cultivation is performed in a cultivation media in a dark condition at a temperature between 25° C. to 30° C. A non-selection media with an antibiotic is introduced. A selection media containing an active ingredient phosphinothricin (PPT) is introduced in a light condition at a temperature of between 25° C. to 30° C. in a sub culture for a period of between 3 weeks to 1 month. The putative transformant is regenerated and the number of copies of the transgenes is analyzed.